Ai9 tdtomato reporter mice
Web007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG … WebThe Jackson Laboratory αsmacreert2 ai9 col2 3 gfp αsmacreert2 Ai9 Col2 3 Gfp, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Ai9 tdtomato reporter mice
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WebBars = 200 μm. d Lung and kidney showed a low level of TdTomato reporter expression in 10-kb Dmp1-Cre(+) mice compared to Cre(−) littermates. The expression was seen in only a small subset of ... WebApr 8, 2024 · Myoblast determination protein 1 (MyoD) dynamics define the activation status of muscle stem cells (MuSCs), aiding in muscle tissue regeneration after injury. However, the lack of experimental platforms to monitor MyoD dynamics in vitro and in vivo has hampered the investigation of fate determination and heterogeneity of MuSCs. Herein, …
WebAi9 mouse (BCM) Official Name: B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J: Species: Mouse: Type: Animal: ... CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair : Annotated Map: WebJan 5, 2024 · The Ai9 reporter mice harbors a lox-STOP-lox (LSL)-tdTomato reporter knockin at the Rosa26 locus 11 (Figure 1). Using two sgRNAs flanking LSL, Cas9 will delete the STOP cassette by NHEJ, resulting in tdTomato expression. 12 This system allows direct detection of cells edited by CRISPR in vivo.
WebThe ratio of the reporter to the reporter control in ESCC mice was also significantly higher than in healthy control mice (P<0.0001; Figure 5C and G). Moreover, the ROC curve … WebAi9 was a gift from Hongkui Zeng (Addgene plasmid # 22799 ; http://n2t.net/addgene:22799 ; RRID:Addgene_22799) For your References section: A robust and high-throughput Cre …
WebStudies in our own laboratory using the Ai9 TdTomato reporter line have shown Dmp1-Cre expression in some osteoblasts, skeletal muscle, a subset of cells in the marrow, stomach, small intestine, skin, brain, eye and an even more restricted subset of cells in the lung and kidney (see Fig 2).
WebApr 4, 2024 · Mutation details: The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing … systemd active failedWebMar 14, 2024 · Before performing triple labeling (PAX2-GlyT2-cellular marker) we wanted to check the specificity of tdTomato expression in the Pax2:Cre-tdTomato transgenic … systemd active exitedWeb目的构建稳定表达的tdTomato转基因小鼠并建系,观察其细胞及组织的荧光表达水平以及其干细胞共培养后的荧光表达水平。方法使用Gateway clone技术和DNA显微注射法构建含有tdTomato表达载体的受精卵,移植至假孕母鼠体内,待其自然生产。使用双向鉴定法鉴定并挑取饲养阳性小鼠并建系。 systemd activestateWeb口腔医学小站 口腔文献阅读与资源分享. 2024年4月3日 发表者 kimi1006 暂无评论. Programmable protein delivery with a bacterial contractile injection system Nature systemd active: inactive deadWebApr 6, 2024 · Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues. systemd aria2cWebAi9 Reporter Mouse, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … systemd analyze securityWebApr 10, 2024 · Ad5-knob(RGD/PK7) produce tdTomato signal in the hippocampus of Ai9 (loxP-tdTomato) mice. Fluorescence was abolished when the spike tip protein (Pvc10) was deleted, confirming that the observed activity was mediated by the ... For assays involving co-transfection of a Cre reporter plasmid or a guide. RNA plasmid, DNA was transfected … systemd analyze