WebNucleic acids, compositions, and methods that allow for the excision of one or more loci from the genome of a host cell are provided herein. In particular, provided herein is an e WebGrindl W, Wende W, Pingoud V, Pingoud A (1998) The protein splicing domain of the homing endonuclease PI-SceI is responsible for specific DNA binding. Nucleic Acids Res 26:1857–1862 CrossRef PubMed CAS Google Scholar Gruen M, Chang K, Serbanescu I, Liu DR (2002) An in vivo selection system for homing endonuclease activity. Nucleic …
Meganuclease - Wikipedia
Webof PI-SceI, the endonuclease domain, which is struc-turally homologous to other homing endonucleases from the LAGLIDADG family, harbors the endo-nucleolytic center of PI … Web20 apr. 2011 · Here we demonstrate that a synthetic genetic element, consisting of mosquito regulatory regions 10 and the homing endonuclease gene I-SceI 11, 12, 13, can … show cps
an, dass inaktive Varianten oder - Translation into English
Web7 jan. 2011 · I-SceI is a monomeric homing endonuclease that recognizes and cleaves within an 18-bp target. It tolerates limited degeneracy in its target … Elucidating how … Web26 mrt. 2004 · Engineering Variants of the I-SceI Homing Endonuclease with Strand-specific and Site-specific DNA-nicking Activity 2008, Journal of Molecular Biology Show abstract Identification of a Single HNH Active Site in Type IIS Restriction Endonuclease Eco31I 2007, Journal of Molecular Biology Show abstract Web2 sep. 2012 · At low endonuclease expression levels, we observed a 25-fold increase in total gene disruption between I-SceI and I-SceI coupled to Trex2 (2.2% to 50.2%, respectively), and nearly 100% of... show cps minecraft