WebHowever, OsGS1S cannot be directly used for developing PPT-tolerant plants because of its poor PPT-resistance. Thus, we performed DNA mixed about OsGS1S press one highly PPT-resistant mutant the deviations in four amino acids (A63E, V193A, T293A and R295K) was isolated after three rounds of DNA shuffling and screening. WebHygromycin B (HygB, Wako Pure Chemical Industries, Osaka, Japan) was added to YPD to a final ... module with the APT gene for G418-resistance, was introduced after every deletion in order to excise the HPT gene. The resulting strains were then grown in non-selective YPD to allow loss of pCU563.

Human CHO-K1/Gα15 Assay - Creative Biolabs

WebResistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). The concentration for selection ranges from 100-1000 µg/ml (typically 200 … Web15 uur geleden · The mannanase is a potentially important target gene may be further exploited to improve abiotic stress resistance in plants. Skip to main content. ... (MS) (containing 20 mg/L of hygromycin) solid medium to screen for positive transformants. The Arabidopsis seedlings of various lines capable of rooting on the hygromycin-containing ... phidias alexander fleming

Hygromycin B Selection Antibiotic InvivoGen

WebDOI: 10.17660/ActaHortic.1998.461.46. Abstract: A series of improved vectors have been constructed that are suitable for use in Agrobacterium tumefaciens -mediated monocot transformation. These binary vectors have several useful features, including the selectable marker genes bar (phosphinothricin resistance) or hph (hygromycin resistance ... WebHygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo. Constitutive expression of the bacterial hyg R gene in … WebTransformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers … phidias athena statue